The combination of the robust phosphoramidite oligonucleotide chemistry and a very high contrast ratio light projector enables us to routinely achieve very high spot uniformity. With our technology, "doughnuts" spots belong in the past.

In the following experiment, spots on a microarray (45-mer probe) were hybridized with a synthetic Cy3-labeled target. The upper panel shows spots as viewed in GenePix Pro 6.0 software, as well as a cross-section of a spot (5 microns resolution). The lower panels show 3-dimensional views of the same spots. Our in situ synthesis technology produces highly uniform spot morphology with very sharp edges.

This translates into high quality microarray images with very high signal to noise ratio using a scanner's full dynamic range. Spots are evenly spaced in both dimensions and accurately fit the software's microarray analysis grid for easy quantification.

Below is a an image from a typical experiment where RNA extracted from a bacterial mutant strain and its wild type counterpart were fluorescently labeled with Alexa fluors (555 and 647, respectively) and cohybidized on an array of 45-mer probes designed against the transcriptome of this bacteria. Click here to open a high-resolution full size image in a new window (12MB). The banner at the top of the page is also another example of a two color experiment performed with MYcroarray.com slides.