Predesigned Target Capture Kits

Don't need a custom design? We offer several types of affordable, predesigned catalog kits for certain applications! To place an order, get a quote, or inquire about kit options, please email us.

Mitochondrial MYTObaits Ultraconserved Elements (UCEs) Whole Genome Enrichment Human Cancer Exome


Ultraconserved elements (UCEs)

As defined by ultraconserved.org, ultraconserved elements (UCEs) are highly conserved regions of organismal genomes shared among evolutionary distant taxa. One can collect data from UCEs and the DNA adjacent to UCE locations (flanking DNA) using target enrichment of UCEs and sequencing. These data are useful for reconstructing the evolutionary history and population-level relationships of many organisms. See publications for several examples. The ultraconserved.org team has designed several sets of baits to capture UCEs in various taxa (see "UCEs" designs below) and permitted us to distribute them. For a current protocol to use the UCE baits, please consult the ultraconserved.org protocols page.

Available Kits

Category I

UCEs: Insect Hymenoptera 2.5K version 2, Principal

Catalog number: MYbaits_Hymenopts_UCE-2.5Kv2_Full

An expanded version of the original HymV1 design, this set of 31,829 baits (120 mer) targets 2,590 UCE loci from the insect order Hymenoptera (ants, bees, sawflies, and wasps). The principal bait list can be downloaded from http://datadryad.org/resource/doi:10.5061/dryad.89n87. It was first described in: MG Branstetter, JT Longino, PS Ward, BC Faircloth. (In press). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. Methods Ecol Evol (DOI: 10.1111/2041-210X.12742)

UCEs: Insect Hymenoptera 2.5K version 2, Ant-Specific

Catalog number: MYbaits_Hymenopts_UCE-2.5Kv2_Ants

This set of 9,898 baits (120 mer) targets 2,524 UCE loci present in Hymenoptera and 16 nuclear exons (12 genes) commonly sequenced in insects. The UCE baits are a subset of the more diverse "principal" bait set, including baits from ant genomes only. The exon baits were designed from four distantly related ant species. The UCE and exon bait lists can be downloaded from http://datadryad.org/resource/doi:10.5061/dryad.89n87. The bait set was first described in: MG Branstetter, JT Longino, PS Ward, BC Faircloth. (In press). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. Methods Ecol Evol (DOI: 10.1111/2041-210X.12742)

UCEs: Insect Hymenoptera 1.5K version 1

Catalog number: MYbaits_Hymenopts_UCE-1.5Kv1

This set of 2,749 baits (120 mer) targets 1,510 UCEs from the insect order Hymenoptera (ants, bees, sawflies, and wasps). The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, MG Branstetter, ND White, SG Brady. (2015) Target enrichment of ultraconserved elements from arthropods provides a genomic perspective on relationships among Hymenoptera. Mol Ecol Resources 15(3): 489-501. doi: 10.1111/1755-0998.12328

UCEs: Tetrapods 5K version 1

Catalog number: MYbaits_Tetrapods-UCE-5Kv1

This set of 5,472 baits (120 mer) targets 5,060 UCEs from tetrapods/amniotes. The 5k probes are known to enrich ~4k loci in birds and reptiles, 2-3k loci in mammals, and up to 1k loci in amphibians. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, JE McCormack, NG Crawford, MG Harvey, RT Brumfield, TC Glenn. (2012) Ultraconserved elements anchor thousands of genetic markers for target enrichment spanning multiple evolutionary timescales. Syst Biol 61:717-726. pmid: 22232343

UCEs: Tetrapods 2.5K version 1

Catalog number: MYbaits_Tetrapods-UCE-2.5Kv1

This set of 2,560 baits (120 mer) targets 2,386 UCEs from amniotes. The 2.5k probes are known to enrich ~2.2k loci in birds and reptiles and ~1k loci in mammals. These baits have not been tested extensively across all tetrapods. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, JE McCormack, NG Crawford, MG Harvey, RT Brumfield, TC Glenn. (2012) Ultraconserved elements anchor thousands of genetic markers for target enrichment spanning multiple evolutionary timescales. Syst Biol 61:717-726. pmid: 22232343. This bait set has also been used for phylogeographic studies by BT Smith, MG Harvey, BC Faircloth, TC Glenn, RT Brumfield. (2013) Target capture and massively parallel sequencing of ultraconserved elements (UCEs) for comparative studies at shallow evolutionary time scales. Syst Biol (In press). doi: 10.1093/sysbio/syt061. pmid: 24021724

UCEs: Ray-finned Fish (Actinopterygians) 0.5K version 1

Catalog number: MYbaits_Actinopts-UCE-0.5Kv1

This set of 2,001 baits (120 mer) targets 500 UCEs from fish. The 0.5k Actinopterygian probes are known to enrich 300-400 loci from ray-finned fishes. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, Sorenson L, Santini F, Alfaro ME. 2013. A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). PLoS ONE 8: e65923. pmid: 23824177

COS: Compositae/Asteraceae 1K version 1

Catalog number: MYbaits_Comp-COS-1Kv1

This set of 9,678 120mer probes targets 1,061 conserved orthologous set (COS) loci in the Compositae/Asteraceae plant family. These ~10K probes are known to enrich >700 loci across a wide range of Compositae species as well as taxa from closely-related families. The bait list can be downloaded here. It was first described in: JR Mandel, RB Dikow, VA Funk, RR Masalia, SE Staton, A Kozik, RW Michelmore, LH Rieseberg, JM Burke. 2014. A Target Enrichment Method for Gathering Phylogenetic Information from Hundreds of Loci: An Example from the Compositae. Applications in Plant Sciences 2(2): 1300085, doi: http://dx.doi.org/10.3732/apps.1300085

Category II

UCEs: Arachnida 1.1K version 1

Catalog number: MYbaits_Arachnid_UCE-1.1kv1

This set of 14,799 baits (120 mer) targets 1,120 UCEs from the Arachnida (spiders, mites, scorpions, harvestmen, and kin). The bait list can be downloaded here. It was first described in: BC Faircloth (2016) Identifying Conserved Genomic Elements and Designing Universal Probe Sets To Enrich Them. In review. BioRxiv doi: 10.1101/077172. The phylogenetic utility of this bait set was demonstrated in: J Starrett, S Derkarabetian, M Hedin, RW Bryson, JE McCormack, BC Faircloth (2016) High Phylogenetic Utility of an Ultraconserved Element Probe Set Designed for Arachnida. In press. Mol Ecol Resources doi: 10.1111/1755-0998.12621.

Pricing

Number of captures * Category I Category II
8 $600 $760
48 $1,920 $2,438
96 $2,880 $3,658
* Please note that the number of reactions displayed here is the recommended number of captures that can be performed, and baits should not be further diluted without prior evaluation. Cat I bait conc. 20ng/µl, Cat II bait conc. 100ng/µl.

We also offer in-house NGS services from library preparation to sequence capture and sequencing!